In this paper, we proposed a new venous valve-like check-valve(V2CV) for microfluidic device which consist of two valve flaps made from PDMS. Compared to conventional check valves in microfluidic devices, this V2CV has simpler structure and easier fabrication, make it possible to be applied in different kinds of microfluidic devices. Experiments were carried out to test its capacity of allowing forward flow and blocking reverse flow.